Adenovirus PCR


Description

Adenovirus is an important pathogen in both the immunocompetent and immunocompromised host. Infections that can be investigated using this real-time, quantitative polymerase chain reaction (PCR) assay include conjunctivitis, keratoconjunctivitis, upper and lower respiratory tract infection, diarrhoea (and vomiting), myocarditis and haemorrhagic cystitis in children. In the immunocompromised, this assay is used for the regular screening of patients, for the early detection of viraemia. Clinical manifestations in such patients include pneumonia, hepatitis, colitis, meningoencephaliltis and disseminated disease. Viral load can be monitored to evaluate the success of treatment with antivirals or CTLs. This assay has important infection control applications, namely through the rapid diagnosis of respiratory or gastrointestinal infection. Additionally, this assay is used for the environmental screening of cubicles, to determine whether adequate disinfection has removed adenovirus from the environment, thus preventing further transmission. The assay includes an internal control for control of both the nucleic extraction and amplification processes.

Also known as


Request a test

To request this test please send sample with a request providing patient ID (three identifiers), specimen information, assay required, relevant clinical details and sender information. Before sending sample please read details on requesting and labelling by clicking on the link. Please also refer to any additional information provided for this test.


Additional information

This assay is duplexed with CMV PCR


Sending address

Microbiology, Virology and Infection Control Level 4, Camelia Botnar Laboratories Great Ormond Street Hospital for Children Great Ormond Street London WC1N 3JH DX number: DX6640203 DX Exchange: Bloomsbury 91WC

Laboratory service

Microbiology, Virology and Infection Control


Sample requirements

Sterile container containing any of the following, as appropriate: EDTA whole blood or plasma (2ml in appropriate blood tube), serum (5ml in clotted blood tube), urine (1ml), NPA, BAL, post-mortem biopsy of any relevant organ, stool, CSF (minimum 200ul) eye swab, nose and throat swab, throat swab (swabs in viral transport medium, available on request). Environmental swabs and the appropriate medium are available on request. Please phone lab to arrange transport.


Reference range

Not applicable


Turnaround time

24 hours


Disease / group

Viral infection


Cost

Upon request


Call in advance?

Please call in advance for environmental swab kit


Microbiology, Virology and Infection Control

Our laboratory performs a wide range of routine and specialist investigations in Bacteriology, Virology and PCR.

© 2011 Great Ormond Street Hospital for Children NHS Trust