Bordetella pertussis real-time PCR


Rapid diagnosis of B. pertussis infection is essential for patient management and infection control. This bacterium has fastidious growth requirements and laboratory culture is slow. Detection of B. pertussis genomic DNA by PCR is rapid and the preferred method of detection for this organism. The assay includes an internal control PCR

Also known as

B. pertussis PCR, pertussis PCR, whooping cough PCR

Request a test

To request this test please send sample with a request providing patient ID (three identifiers), specimen information, assay required, relevant clinical details and sender information. Before sending sample please read details on requesting and labelling by clicking on the link. Please also refer to any additional information provided for this test.

Additional information

Multiplexed assay that detects two B. pertussis genomic targets.

Sending address

Microbiology, Virology and Infection Control Level 4, Camelia Botnar Laboratories Great Ormond Street Hospital for Children Great Ormond Street London WC1N 3JH DX number: DX6640203 DX Exchange: Bloomsbury 91WC

Laboratory service

Microbiology, Virology and Infection Control

Sample requirements

Nasopharengeal aspirates (NPA) and pernasal swabs (PNS). Minimum volume 200 microlitres

Reference range

Not applicable

Turnaround time

24 hours

Disease / group

Bacterial infection


Upon request

Call in advance?

Not required

Microbiology, Virology and Infection Control

Our laboratory performs a wide range of routine and specialist investigations in Bacteriology, Virology and PCR.

© 2011 Great Ormond Street Hospital for Children NHS Trust