Detection of Clostridium difficile toxin


Clostridium difficile causes a spectrum of disease ranging from antibiotic-associated diarrhoea to pseudomembranous colitis. This assay detects the bacterial toxin by detection of the disruption of a cell monolayer. Neutralisation of the toxin is used to confirm the result. This sensitive assay is used as part of the screening protocol for bone marrow transplant patients, as well as a diagnostic tool in patients presenting with loose stools. Given the potential for nosocomial outbreaks with this organism, the results obtained using this reliable assay are exceptionally useful for the infection control team, not just individual patients.

Also known as

C. diff, C. difficile

Request a test

To request this test please send sample with a request providing patient ID (three identifiers), specimen information, assay required, relevant clinical details and sender information. Before sending sample please read details on requesting and labelling by clicking on the link. Please also refer to any additional information provided for this test.

Additional information

Not applicable

Sending address

Microbiology, Virology and Infection Control Level 4, Camelia Botnar Laboratories Great Ormond Street Hospital for Children Great Ormond Street London WC1N 3JH DX number: DX6640203 DX Exchange: Bloomsbury 91WC

Laboratory service

Microbiology, Virology and Infection Control

Sample requirements

Stool (walnut sized amount in sterile container)

Reference range

Not applicable

Turnaround time

2 days

Disease / group

Bacterial infection


Upon request

Call in advance?

Not required

Microbiology, Virology and Infection Control

Our laboratory performs a wide range of routine and specialist investigations in Bacteriology, Virology and PCR.

© 2011 Great Ormond Street Hospital for Children NHS Trust