Kingella kingae real-time PCR


Primary osteoarticular infections (OAI) in children must be diagnosed and treated urgently because of the risk of prolonged morbidity and crippling long-term sequelae. Isolation of the cuasative organism is the traditional way to confrim diagnosis. K. kingae is now considered to be the leading cause of OAI in young children. However, it�s prevalance in OAI is underestimated by culture due to its fastidious nature. PCR based methods improve the diagnosis of K. kingae OAI and a specific-real time PCR is more sensitive than broad-range 16S rDNA PCR. The assay includes an internal control PCR.

Also known as

K. kingae PCR, kingella PCR

Request a test

To request this test please send sample with a request providing patient ID (three identifiers), specimen information, assay required, relevant clinical details and sender information. Before sending sample please read details on requesting and labelling by clicking on the link. Please also refer to any additional information provided for this test.

Additional information

This assay is performed as part of a multiplex with S. pneumoniae

Sending address

Microbiology, Virology and Infection Control Level 4, Camelia Botnar Laboratories Great Ormond Street Hospital for Children Great Ormond Street London WC1N 3JH DX number: DX6640203 DX Exchange: Bloomsbury 91WC

Laboratory service

Microbiology, Virology and Infection Control

Sample requirements

Any sample from a normally sterile site. Minimum volume 200 microlitres

Reference range

Not applicable

Turnaround time

24 hours

Disease / group

Bacterial infection


Upon request

Call in advance?

Not required

Microbiology, Virology and Infection Control

Our laboratory performs a wide range of routine and specialist investigations in Bacteriology, Virology and PCR.

© 2011 Great Ormond Street Hospital for Children NHS Trust