Quantiferon TB Gold assay


Tuberculosis infection due to organisms belonging to the Mycobacterium tuberculosis complex can be symptomatic or latent, both of which can be detected by this assay. The assay tests for cell mediated immune responses to peptide antigens that stimulate the mycobacterial proteins ESAT-6, CFP-10 and TB7.7(p4). Patients infected with a M. tuberculosis complex organism normally have lymphocytes in their blood that recognise these antigens. This recognition process involves the generation and secretion of the cytokine, IFN-g. The detection and subsequent quantification of IFN-g by enzyme-linked immunosorbent assay (ELISA) forms the basis of this test. Results are used in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.

Also known as


Request a test

To request this test please send sample with a request providing patient ID (three identifiers), specimen information, assay required, relevant clinical details and sender information. Before sending sample please read details on requesting and labelling by clicking on the link. Please also refer to any additional information provided for this test.

Additional information

Please refer to the instructions regarding blood collection which accommpany the collection tubes (available on request)

Sending address

Microbiology, Virology and Infection Control Level 4, Camelia Botnar Laboratories Great Ormond Street Hospital for Children Great Ormond Street London WC1N 3JH DX number: DX6640203 DX Exchange: Bloomsbury 91WC

Laboratory service

Microbiology, Virology and Infection Control

Sample requirements

1ml of blood in each of the three tubes provided (fill to black mark on the side of each tube, in the order grey tube, red tube, purple tube). Mix the blood thoroughly in each tube once collected. Transport to the laboratory ASAP (within 16 hours), keeping the tubes upright. If transport is delayed, store blood at room temperature. Do not refrigerate or freeze.

Reference range

Not applicable

Turnaround time

7 days

Disease / group

Mycobacterial infection


Upon request

Call in advance?

Please call in advance if urgent

Microbiology, Virology and Infection Control

Our laboratory performs a wide range of routine and specialist investigations in Bacteriology, Virology and PCR.

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