Staphylococcus aureus real-time PCR


Staphylococcus aureus is a catalase-positive, gram-positive coccus that may form part of the normal flora of the skin and other sites such as the upper respiratory tract. S. aureus causes a wide range of major and minor infections, including wound infections, abscesses, bacteraemia, osteomyelitis, pneumonia and endocarditis. Production of the enzyme coagulase is its main distinctive diagnostic feature. Molecular methods of detection have targeted a range of S. aureus specific genes, this assay uses the Coa gene (Coagulase enzyme) and the Sa442 fragment. The identification of methicillin-resistant S. aureus (MRSA) is based on detection of the mecA gene. The assay includes an internal control PCR

Also known as

S. aureus PCR, Staph aureus PCR

Request a test

To request this test please send sample with a request providing patient ID (three identifiers), specimen information, assay required, relevant clinical details and sender information. Before sending sample please read details on requesting and labelling by clicking on the link. Please also refer to any additional information provided for this test.

Additional information

This assay is performed as part of a multiplex with S. pyogenes.

Sending address

Microbiology, Virology and Infection Control Level 4, Camelia Botnar Laboratories Great Ormond Street Hospital for Children Great Ormond Street London WC1N 3JH DX number: DX6640203 DX Exchange: Bloomsbury 91WC

Laboratory service

Microbiology, Virology and Infection Control

Sample requirements

Any sample from a normally sterile site. Respiratory samples. Minimum volume 200 microlitres

Reference range

Not applicable

Turnaround time

24 hours

Disease / group

Bacterial infection


Upon request

Call in advance?

Not required

Microbiology, Virology and Infection Control

Our laboratory performs a wide range of routine and specialist investigations in Bacteriology, Virology and PCR.

© 2011 Great Ormond Street Hospital for Children NHS Trust